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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Tomey Corporation inbuilt software of the casia 2 as-oct ss-1000
Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence <t>Microscope</t> inbuilt software (Model <t>BZ-X710,</t> KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.
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Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence Microscope inbuilt software (Model BZ-X710, KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.

Journal: Scientific Reports

Article Title: Common molecular profile of multiple structurally distinct warfare arsenicals in causing cutaneous chemical vesicant injury

doi: 10.1038/s41598-024-83513-1

Figure Lengend Snippet: Effects of structurally different arsenicals on apoptotic cell death. ( A ) TUNEL assay of skin sections from vehicle- or treated mice revealed abundant TUNEL-positive (green) cells in DPCA and DPCYA-treated skin, indicating robust cell death (Yellow arrows) at 24 and 72 h. Scale bar-50 µm. ( B ) Histogram showing quantitative analysis of TUNEL-positive green cells. Keyence Microscope inbuilt software (Model BZ-X710, KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells. Multiple microphotographs captured at 10X magnification was used for quantitative analysis. ( C ) Western blot analysis shows augmented expression of cleaved caspase 3 in arsenical-challenged mice. β-actin was used as an endogenous control. (D) Histogram representing densitometry analysis of western blots band intensity. (Also see supplementary fig. S4, S5 and S6 for full images of immunoblots). *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns, non-significant. N = 3/group.

Article Snippet: Keyence Microscope inbuilt software (Model BZ-X710, KEYENCE, Osaka, Japan) was used for quantitative analysis of TUNEL-positive cells.

Techniques: TUNEL Assay, Microscopy, Software, Western Blot, Expressing, Control